International Journal of Applied Science - Research and Review Open Access

ZmCCD8b encodes a carotenoid cleavage dioxygenase regulating the plant response to phosphorus limitation

EuroSciCon Joint Events on Plant Science, Tissue Engineering and Parasitology

December 03-04, 2018 Amsterdam, Netherlands

Zhong Yanting, Xiaoying Pan, Ruifeng Wang, Faisal Nadeem, Jianyu Zhao, Yanjun Xu, Xuexian Li

China Agricultural University, China

Posters & Accepted Abstracts: Int J Appl Sci Res Rev

Abstract:

In our study, overexpression of ZmCCD8b, a paralogous gene of ZmCCD8a, in Arabidopsis mutant max4 failed to rescue the mutant phenotype. However, we found ZmCCD8b expression is significantly higher in silk, while relative higher in ear and root under low phosphorus (LP) condition reciprocal to control (sufficient nutrients) reaching the highest level on the tenth day. The expression was stronger in pericycle of the meristematic zone and the elongation zone as revealed by In situ hybridization. Protoplast amplification showed that this gene was localized to the plastid. We used virus induced gene silencing (VIGS) technology to silence ZmCCD8b and the results indicated that in contrast to GFP control, the carotenoid contents significantly reduced in leaves of Zmccd8b lines. In contrast to increased phosphorus concentration in root whereas, decreased phosphorus concentration in shoot, root biomass of Zmccd8b silenced lines non-significant. While shoot dry weight decreased significantly as compared to GFP control, contributing to an increased root to shoot ratio of Zmccd8b silenced lines. Furthermore, ZmCCD8b may affect the expression of PHO2 and PHO1 through PHRs. Additionally, ZmPht1;1 and ZmPht1;6 were down-regulated whereas, ZmPht1;3 and ZmPht1;13 were upregulated in Zmccd8b silenced lines under LP conditions. Yeast one-hybrid and EMSA experiments verified that ZmPHR1s can regulate ZmCCD8b through P1BS element. Transcriptome analysis indicated increased expression of genes related to stress and signal transduction in ZmCCD8b heterologous overexpression lines.

Biography :

Zhong yanting, is pursuing his PhD from College of resources and environmental sciences, China Agricultural University, Beijing, China. His research broadly focuses on Molecular Biology of Plant Nutrition, and currently working on Phosphorus .

E-mail: zhongyanting93@163.com