Saeed El-Ashram, Xue Wang and Jiangdong Wu
Foshan University, China
Posters & Accepted Abstracts: J Clin Gastroenterol Hepatol
Bacillus cereus is a Gram-positive, spore-forming associated with food contamination. To our knowledge, no studies have been reported regarding transcriptome analysis of duckling spleen in the context of B. cereus. In this study, we carried out RNAseq- based transcriptome analysis on the spleen of ducklings. Six 1-day-old Muscovy ducklings were equally divided into infected and uninfected control groups with three replicates per group. Ducks in the infected group were inoculated with 105 CFU B. cereus by oral gavage individually. The control group was administered with sterile phosphate buffer saline. Ducks were euthanatized and the spleen was collected on 24 h post-infection (HPI). Key genes upregulated in response to B. cereus infection were 60S ribosomal protein L34 (RPL34) and small conductance calcium-activated potassium channel protein 2 (KCNN2) in comparison between the Bacillus-infected and uninfected control ducklings. However, five genes were substantially repressed between the aforementioned groups. GO term enrichment analysis suggested that up-regulated and down-regulated DEG significantly enriched in biological processes and molecular functions. Our findings have indicated that multiple genes and pathways may play a crucial role in response to Bacillus infection within 24 HPI, offering candidate targets and strategies for prevention and control.
E-mail:
saeed_elashram@yahoo.com
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