Physical exercise prevents memory impairment in an animal model of hypertension through modulation of CD39ÃƒÂ¾ and CD73ÃƒÂ¾activities and A2A receptor expression

Andre’ ia Machado Cardoso; Le; ro Henrique Manfredi; Daniela Zanini; Margarete Dulce Bagatini; Jessie’ Martins Gutierres; Fabiano Carvalho; Alain Tremblay; Adriane Bello ’Klein; Maribel Antonello Rubin; Vera Maria Morsch; Jean Se’vigny; Maria Rosa Chitolina Schetinger

doi:10.21767/2471-8157-C1-006

Physical exercise prevents memory impairment in an animal model of hypertension through modulation of CD39^ÃÆÃÂ¾ and CD73^ÃÆÃÂ¾activities and A_2A receptor expression

2^nd Edition of EuroSciCon Congress on Heart Disease and Interventional Cardiology

February 25-26, 2019 | Paris, France

Andre’ ia Machado Cardoso, Leandro Henrique Manfredi, Daniela Zanini, Margarete Dulce Bagatini, Jessie’ Martins Gutierres, Fabiano Carvalho, Alain Tremblay, Adriane Bello ’Klein, Maribel Antonello Rubin, Vera Maria Morsch, Jean Se’vigny, and Maria Rosa Chitolina Schetinger

Federal University of Fronteira Sul, Campus Chapeco’, Brazil CCNE-Federal University of Santa Maria, Brazil Universite de Montreal, Brazil Centre de recherche du-CHU de Que’ bec, Universite’ Laval, USA Health Basic Sciences Institut-UFRGS, Brazil

Posters & Accepted Abstracts: Interv Cardiol J

Abstract:

Central nervous system function has been emerging as an approach to understand hypertension-mediated memory dysfunction, and chronic exercise is able to modulate the purinergic system. Herein, we investigated the effect of chronic swimming training on the purinergic system in cortex and hippocampus of L-NAME-induced hypertensive rats. Male Wistar rats were divided into four groups: control, exercise, L-NAME and exercise L-NAME. Inhibitory avoidance test was used to assess memory status. NTPDase, CD73þ and adenosine deaminase activities and expression, and P2 receptors expression were analyzed. Data were analyzed using two-way ANOVA and Kruskal–Wallis tests, considering P less than 0.05. Physical exercise reduced the blood pressure and prevented memory impairment induced by L-NAME model of hypertension. L-NAME treatment promoted an increase in NTPDase1, NTPDase3 and CD73þ expression and activity in the cortex. A_2A expression is increased in hippocampus and cortex in the hypertension group and exercise prevented this overexpression. These changes suggest that hypertension increases adenosine generation, which acts through A_2A receptors, and exercise prevents these effects. These data may indicate a possible mechanism by which exercise may prevent memory impairment induced by L-NAME.