Insights in Analytical Electrochemistry Open Access

Molar concentration welcomes Avogadro in postgenomic analytics

Joint Event:8^th Annual Congress on Analytical and Bioanalytical Techniques & 14^th International Conference and Exhibition on Pharmaceutical Formulations

August 28-30, 2017 Brussels, Belgium

A I Archakov, A V Lisitsa and E A Ponomarenko

Institute of Biomedical Chemistry, Russian Federation

Scientific Tracks Abstracts: Insights Anal Electrochem

Abstract:

The researchers working with high-throughput methods of genomics, transcriptomics, and proteomics reconsider the concept of concentration and evaluate the data obtained in the number of copies of biomacromolecules. Measurement of copy number reflects a steady trend in increasing the sensitivity of postgenomic analytical methods, up to the level of a single molecule. In this paper we review the physical meaning of the terms "molar concentration" and "Avogadro��?s number" to establish a relationship between them. The relationship between the molar concentration and the number of copies of that same macromolecule in a certain volume is set through the reverse Avogadro��?s number, the value of which (��?10-24 �?) characterizes the molar concentration of a single molecule in 1 liter. Using the reverse Avogadro��?s number, we deal with situations in analyzing homogeneous biological solutions and heterogeneous cellular material.