American Journal of Advanced Drug Delivery Open Access

Gold Aryl nanoparticles coated with polyelectrolytes for adsorption and protection of DNA against nuclease degradation

Joint Event on 31^st Nano Congress for Future Advancements & 13^th Edition of International Conference on Nanomedicine and Advanced Drug Delivery

August 29-30, 2019 London, UK

Seema Panicker, Islam M. Ahmady and Ahmed A. Mohamed

University of Sharjah, UAE

Posters & Accepted Abstracts: Am J Drug Deliv

Abstract:

Binding DNA on nanoparticles was pursued to form nanoplatform for formation of nonâ�?viral gene system. Carboxyl derivatized goldâ�?aryl nanoparticles can bind with biodegradable cationic polyelectrolytes such as polydiallyldimethylammonium chloride (PDADMAC). In our study, we used goldâ�?aryl nanoparticles (AuNPs) treated with PDADMAC to form conjugates with nonâ�?thiol or nonâ�?disulfide modified oligonucleotide DNA. Both AuNPsâ�?DNA and PDADMACâ�?AuNPsâ�?DNA biomaterials were characterized using UV–Vis, dynamic light scattering (DLS), atomic force microscopy (AFM), transmission electron microscopy (TEM) and agarose gel electrophoresis. UV–Vis showed a red shift in the plasmon peak as compared with unconjugated AuNPs. DLS measurements also showed difference in the size of AuNPsâ�?DNA and PDADMACâ�?AuNPsâ�?DNA. AFM and TEM results showed proper conjugation of DNA with AuNPs. Gel electrophoresis proved the presence of interaction between PDADMACAuNPs and negatively charged DNA. The binding of DNA in the described bioconjugate enhanced its protection against nuclease degradation and prolonged its presence in the digestive environment of DNaseâ�?I. From the results we expect that these biomaterials can be used in nanomedicine with emphasis on nonâ�?viral gene system.