Trends in Green Chemistry Open Access

Evaluation of novel thermo tolerant haloalkaliphilic bacterium Halomonas stevensii for biomitigation of gaseous phase CO2: Energy assessment and product evaluation studies

Joint Event on 8^th International Conference on Environmental Chemistry and Engineering & 7^th Edition of International Conference on Green Energy, Green Engineering and Technology

September 20-22, 2018 Berlin, Germany

Somesh Mishra

Indian Institute of Technology Delhi, India

Posters & Accepted Abstracts: Trends in Green chem

Abstract:

Present work deals with the bio-mitigation potential of gaseous phase CO2 by chemolithotrophic bacterium Halomonas stevensii isolated from haloalkaliphilic habitat using thiosulfate ion (S2O32-) as an energy source. H. stevensii was tested for various abiotic stress tolerance such as salt [1 - 14% (%w: v)], temperature (35-80oC) and pH (4-12). Batch studies were conducted for 6 days at 15 (±1) % (% v:v) inlet CO2 concentration to find the CO2 fixing capability of H. stevensii under varying concentration of energy substrate i.e. 0, 50 and 100 mm Na2S2O3. Approximately 98% CO2 removal from gaseous phase was achieved at 50 and 100 mm Na2S2O3. Biomass productivity was estimated in terms of maximum biomass productivity (P-Max) and specific growth rate (μMax). 6th day sample (biomass and supernatant) obtained from 100 mm Na2S2O3 batch study was characterized by FTIR and GC-MS to identify the products formed from CO2 fixation. The evaluation of CO2 fixation by H. stevensii into primary metabolite was carried out by growing the H. stevensii at 5%, 10% and 15 % (% v: v) inlet CO2 concentration for the duration of 6 days. The obtained leachate was further analyzed by GC for the quantification of fatty alcohols. The utilization of gaseous phase CO2 by H. stevensii is also proven by conducting the approximate materials balance and energy assessment for the present CO2 fixation process.

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