Insights in Analytical Electrochemistry Open Access

Effect of laser-induced shockwave on molecules and particles in solution

Joint Event:8^th Annual Congress on Analytical and Bioanalytical Techniques & 14^th International Conference and Exhibition on Pharmaceutical Formulations

August 28-30, 2017 Brussels, Belgium

Nobuyuki Ichinose

Kyoto Institute of Technology, Japan

Scientific Tracks Abstracts: Insights Anal Electrochem

Abstract:

Focusing of a nanosecond laser pulse (â�?�?200 �?¼J) into aqueous solutions with an objective lens generates a high temperature plasma by dielectric breakdown, which induces generation of shockwave. Propagation of the shockwave with a high pressure causes linear and non-linear effects on the solute or dispersed particles. Time-resolved fluorescence spectroscopic observation under microscope has revealed that the shockwave affects local concentration of solutes due to a sub-mm movement of the molecules/particles with a near-sonic velocity in water. Combination of a â�?�?100�?¼m capillary to confine the shockwave propagation into one-dimension and a collagen gel to control the holding and releasing of the loaded molecules/particles made their movement give a spot as if they were brought by a laminar flow. The distance travelled of a few tens to hundreds �?¼m by the fluorescent-labeled proteins, DNAs, and polysaccharides or CdSe nanoparticles was found to be molecular type- and sizedependent. This technique (laser-induced shockwave chromatography) can avoid unwanted adhesion onto the solid stationary phase and will be applicable to prompt analysis to study aggregation/polymerization phenomena of biomolecules.

Biography :

Nobuyuki Ichinose received his PhD from Osaka Prefecture University, Japan. He is the professor of Kyoto Institute of Technology, Japan. He has over 60 publications in various fields in chemistry.