American Journal of Advanced Drug Delivery Open Access

Biosynthesis and anticancer effects against colon cancer cells of silver nanoparticles using Jurenia macrocephala DC. whole extract

Joint Event on 31^st Nano Congress for Future Advancements & 13^th Edition of International Conference on Nanomedicine and Advanced Drug Delivery

August 29-30, 2019 London, UK

Madhulika Bhagat, Monica Sangral, Rythem Anand, Ajay Kumar and Pooja Sharma

University of Jammu, India

Posters & Accepted Abstracts: Am J Drug Deliv

Abstract:

Metal nanoparticles, particularly silver nanoparticles (AgNPs), are developing more important roles as diagnostic and therapeutic agents for cancers with the improvement of eco-friendly synthesis methods. This study demonstrates the biosynthesis, antibacterial activity, and anticancer effects of silver nanoparticles using Jurenia macrocephala DC. (Family Compositae) whole aqueous extract. The AgNPs were characterized by UV-vis absorption spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and Fourier transform infrared spectroscope (FTIR). The cytotoxicity effect was explored on human colon cancer cells in vitro by MTT assay. The expressions of phosphorylated stat 3, bcl-2, survivin, and caspase-3 were examined by Western blot analysis. The whole extract acted as a strong reducing and stabilizing agent during the synthesis. AgNPs also showed dose-dependent cytotoxicity against colon cancer cells through a decrease of stat 3, bcl-2, and survivin, as well as an increase in caspase-3. These findings confirm the potential anticancer application of AgNPs for colon cancer therapy. Further research should be focused on the comprehensive study of molecular mechanism and in vivo effects on the colon cancer.