Department of primary Health, Yunnan University, Kunming, China
Research Article
The Novel circRNA hsa_circ_0000038 Inhibits the Progression of Hepatocellular Carcinoma by Sponging miR-92a-2-5p to Regulate the p53/p21 Proteins
Author(s): Sihang, Mengting Luo, Zhengyuan Zeng, Lei Shen, Renchao Zou, Jia Wei*, Taicheng Zhou and Qian Feng
Background: The abnormal regulation of circular RNA (circRNA) levels is commonly identified in human diseases, particularly malignant tumors. Recently, the diagnostic value of circRNAs has received increased attention. The detailed mechanisms of various cancer and circular RNAs need more research to clarify, including hepatocellular carcinoma HCC.
Methods: We utilized quantitative Real-Time fluorescence Polymerase Chain Reaction (RT-qPCR) to measure the expression level of hsa_circ_0000038 in paired Hepatocellular Carcinoma (HCC) and adjacent noncancerous liver tissues. GO annotation and enrichment analysis were used to examine the potential downstream pathways. RT-qPCR and Western blotting were conducted to evaluate the expression of thep53/p21pathway. CCK-8, wound closure and Tran’s well assays were used to measure cell proliferation.. View More»
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