Department of Biology, Loyola Institute of Frontier Energy (LIFE), Chennai, India
Research Article
Extraction of Antibacterial Surface-active Molecule from Serratia marcescens MT053078: Characterization and Biological Evaluation
Author(s): Vijayaraj R*
Bio-surfactant and red pigment producing, a special bacterial strain was isolated from oil containing soil sediment sample, collected from the Ennore Coastal Region which is located beside the Coromandel Coast of the Bay of Bengal. The potential strain was identified as Serratia marcescens MT053078 based on the biochemical and physiological tests together with 16S rRNA sequence analysis. Maximum production and culture conditions were optimized with the support of emulsification index test through different parameters such as pH, temperature, rpm, salinity, carbon/ nitrogen sources, metal ions and different edible oils. Extraction, purification and characterization of bio-surfactant succeeded by medium fermentation process in optimized condition. Thin Layer Chromatography (TLC) and Fourier Transform Infrared Spectroscopy (FT-IR) were employed to confirm the class of bio-surfactant as l.. View More»
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