Vijayaraj R*
Bio-surfactant and red pigment producing, a special bacterial strain was isolated from oil containing soil sediment sample, collected from the Ennore Coastal Region which is located beside the Coromandel Coast of the Bay of Bengal. The potential strain was identified as Serratia marcescens MT053078 based on the biochemical and physiological tests together with 16S rRNA sequence analysis. Maximum production and culture conditions were optimized with the support of emulsification index test through different parameters such as pH, temperature, rpm, salinity, carbon/ nitrogen sources, metal ions and different edible oils. Extraction, purification and characterization of bio-surfactant succeeded by medium fermentation process in optimized condition. Thin Layer Chromatography (TLC) and Fourier Transform Infrared Spectroscopy (FT-IR) were employed to confirm the class of bio-surfactant as lipopeptide. A peak of absorption at about 265 nm was detected in the UV-visible spectrum. The emulsification index activity of Cell Free Supernatant (CFS) with kerosene oil was found to be above 80% by the recommended formula. The results proved that the extracted biosurfactant has potential efficacy against clinical pathogens, as evidenced by the antibacterial and antibiofilm activities.
Published Date: 2025-01-21; Received Date: 2023-10-05
