Department of Chemistry, Hormozi Laboratory of Chemistry and Biochemistry, Iran
Research Article
A Comparative Study on Kinetics Performances of BSA-gold Nanozymes for Nanozyme-mediated Oxidation of 3,3?,5,5?-Tetramethylbenzidine and 3,3?-Diaminobenzidine
Author(s): Saeed Reza Hormozi Jangi*
Commonly 3,3'-diaminobenzidine and 3,3',5,5'-thetramethylbenzidine, abbirvated as DAB and TMB, in turn, have
been employed as the standard nanozyme substrates for probing the nanozme-catalyzed oxidation, and the corresponding
oxidation products of these substrates have been probed as the analytical identifiers for sensing/biosensing
application in lab/medial scales. However, it is well-known that the affinity of these substrates toward
binding to enzymes or especially, here, nanozymes is not the same, resulting in different enzyme-like activity and
different kinetics indexes including Km and Vmax. Since, the protein-assisted/protected gold nanozymes (BSA-gold
nanozymes) are type high powerful artificial peroxidase enzymes, Therefore, the kinetics indexes of these nanozymes
including both Km and Vmax should be changed by .. View More»
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