Department of Biotechnology, International Islamic University, Islamabad, 44000, Pakistan
Research Article
Experimentation of the IFAST Technique for DNA Extraction using Two Different Methods
Author(s): Saad Butt*, Muhammad Faisal, Hammad Ali Sajid and Muhammad Imran Shabbir
Purpose: Immiscible Filtration Assisted by Surface Tension (IFAST) is a microfluidic technique that uses the principle of surface tension for the extraction of analyses such as nucleic acids, proteins, and metabolites. This immiscible phase filtration technique was tested via two different cost-effective methods. Methods: Two cost-effective methods for applying the IFAST technique were developed and the procedures were compared for effectiveness. These methods involved the use of plastic canvas and Eppendorf tubes for device fabrication and DNA extraction. Results: In the first method, several challenges are faced, which are discussed in this research, where plastic canvas is used instead of expensive materials such as Polydimethylsiloxane (PDMS). On the other hand, the air-jump method is better for DNA extraction because of the new approach of using Eppendorf tubes for IFAST. Conclus.. View More»
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