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Separation the diastereomers of acyclic isoprenoid alkanes by gas chromatography and its geochemical significance

Joint Event on 9^th Edition of International Conference on Chemistry Science and Technology & 11^th International Conference & Expo on Chromatography Techniques

April 22-24, 2019 Dublin, Ireland

Yingqin Wu

Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, China

Scientific Tracks Abstracts: Arch Chem Res

Abstract:

The abundant acyclic isoprene alkanes, including norpristane (iC18, 2, 6, 10- tetramethyl tetradecane), pristane (Pr, 2, 6, 10, 14- tetramethylpentadecane), phytane (Ph, 2, 6, 10, 14- tetramethylhexadecane) and their diastereomers were detected and separated by GC-MS/MS in the coal sample of the Junggar Basin. Furthermore, the optimized condition for separation was discussed in terms of column type, length, and column temperature program. The results showed that the separation degree (R) of their diastereomers (e.g. 6(R)10(S)), 6(S)10(S)+6(R)10(R)) of iC18, Pr and Ph reached 0.6, 0.8 and 0.9 respectively. It indicated that the method was versatile, simple, rapid and efficient to separate the diastereomers of acyclic isoprene alkanes. The optimum condition for separation are as follows: GC was fitted with a HP-5 capillary column of 100 m × 0.32 mm × 0.25 μm, Agilent, USA with a He as carrier gas set in a split (10:1) injection mode with an injector temperature of 280°C. The GC oven was held isothermal at 80°C (1 min), programmed to 100°C (1 min) at 5°C min-1, from 100°C to 130°C (5 min) at 0.3°C min-1, from 130°C to 280°C at 5°C min-1, and held for 30 min at 280°C.

Results and Conclusions:

1. Evenick J (2016) Evaluating source rock organofacies and paleodepositional environments using bulk rock compositional data and pristane/ phytane ratios. Marine and Petroleum Geology 78:507-515.

2. Wu Y, Xia Y, Wang Y and Lei T (2016) The Geochemical characteristics of coals from the junggar basin in Northwest China and the relation of the configuration of pristane with maturity in highly mature and overmature samples. Oil Gas Sci. Technol. 71(3):35.

3. Dawson K, Schaperdoth I, Freeman K and Macalady J (2013) Anaerobic biodegradation of the isoprenoid biomarkers pristane and phytane. Organic Geochemistry 65:118-126.

4. Xu G, Shuai Y, Wang P, Zhang D, (2010) Chromatographic separation of pristane and phytane stereoisomers and geochemical significance. Geochemistry 39(5):491-496.

5. Peters K, Walters C and Moldowan J (2005) The biomarker guide: biomarkers and isotopes in petroleum system sand earth history. Cambridge: Cambridge University Press, 475-477

Biography :

Wu Yingqin has her major research interests in the application of gas chromatography mass spectrometry technology, organic geochemistry and its implications for resources of oil and gas, environmental geochemistry and environmental pollution remediation and biogeochemistry: activities, mechanisms and cycles. She has built a new method for separating the stereoisomers of acyclic isoprenoid alkanes by gas chromatography and extracting biomarkers from biodegraded heavy oil after years of experience in research.