Journal of Infectious Diseases and Treatment Open Access

Representatives of Firmicutes isolated from goats‘ milk in Slovakia

Joint Event on 6^th World Congress and Expo on Applied Microbiology & 8^th Edition of International Conference on Antibiotics, Antimicrobials & Resistance & 12^th International Conference on Allergy & Immunology

October 21-22, 2019 Rome, Italy

Andrea Laukova, Viola Strompfova, Monika Pogany Simonova, Ivana Kubasova and Anna Kandricakova

Centre of Biosciences, Slovak Academy of Sciences, Slovakia

Posters & Accepted Abstracts: J Infec Dis Treat

Abstract:

Nowadays, nutrition is very popular return to all natural, e.g. also consumption of goats‘/ewes‘milk is increasing; although it has had long-term tradition in Slovakia. Ewes‘ milk contains a lot of minerals, calcium content is even bigger than in cows‘ milk. There are also vitamins involving folic acid and fatty acids such as butyric, capronic, caprilyc, caprine, but also palmic, linolenic and arachidonic acids. Proteins are represented by high percentage of amino acids (threonine, isoleucine, tyrozine, lysine, cystein and valine). Ewes‘ milk has anti-allergic properties and it is easily digestable. Dairy industry in Slovakia uses mostly commercial cultures in dairy goats products. However, to select autochthonous strain for proper use is preferred. This is also the aim of our study. Up to now twentyeight samples of milk from 156 goats were treated using the standard microbiological dilution method (ISO) and the appropriate dilutions were plated on MRS agar (Merck), M-Enterococcus agar (Difco) and MSA agar (Oxoid) to select mostly representatives of the genus Lactobacillus but Enterococcus and Staphylococcus as well. Three strains were belonged to the species Lactobacillus paracasei (LPa 12/1, LPar ZM-1, LPar ZM-2) using MALDI-TOF spectrometry. These strains sufficiently tolerate pH 3; after 90 min cultivation their counts still were not decreased (up to 6.0 log cycle) compared to start of cultivation. They inhibited the principal indicator Enterococcus avium EA5 with inhibition zones up to 24 mm. Among enterococci, four species were determined (Enterococcus faecium, E. faecalis, E. hirae and E. mundtii) and also species variability was demonstrated in coagulase-negative staphylococci (Staphylococcus xylosus, S. warneri, S. pasteuri, S. schleiferi and S. hominis). The other tests are in process including technological properties of L. paracasei strains. Results were achieved in the framework of the project APVV-17- 0028.