Journal of Infectious Diseases and Treatment Open Access

Plantaricin plays a role for post-fermented tea

Joint Event on 6^th World Congress and Expo on Applied Microbiology & 8^th Edition of International Conference on Antibiotics, Antimicrobials & Resistance & 12^th International Conference on Allergy & Immunology

October 21-22, 2019 Rome, Italy

Yolani Syaputri, Masanori Horie and Hitoshi Iwahashi

Gifu University, Japan Health Research Institute, AIST, Japan

Scientific Tracks Abstracts: J Infec Dis Treat

Abstract:

Post-fermented tea samples, before fermentation, primary and secondary fermentation process from each company were collected and studied. Klebsiella pneumoniae subsp. ozaenae, Pseudomonas glareae, Klebsiella variicola, and Pseudomonas aeruginosa were observed in tea leaf before fermentation. Furthermore, in primary and secondary fermentation Lactobacillus plantarum became one of the major lactic acid bacteria species can survive in these processes. The results showed that plnA, plnEF, and plnNJK existed in primary and secondary fermentation. Two potentials L. plantarum were isolated from post-fermented tea samples, L. plantarum IYO1501 and L. plantarum IYO1511. plnA and plnEF were encoded on plasmid of L. plantarum IYO1501 and L. plantarum IYO1511. Whereas, plnNJK was encoded on L. plantarum IYO1511 chromosome. Schematic representation of plnA, plnEF, and plnNJK gene cluster in L. plantarum showed different schematics gene cluster. The cluster structure of plantaricin encoding region was not shown yet around plantaricin gene region before. plnA on L. plantarum IYO1501 showed 96% homology of GenBank. The results showed clearly that L. plantarum IYO1501 and L. plantarum IYO1511 produced plantaricin on plasmid and on chromosome and play an important role for the success of the product.

Recent Publications

1. Strong Antimicrobial of Lactic Acid Bacteria and Species Identification of Virgin Coconut Oil Products in Padang West Sumatera, Indonesia (2017). International Journal on Advance Science, Engineering and Information Technology (IJASEIT)

2. Isolation and Characterization of Lactobacillus spp. From Virgin Coconut Oil, West Sumatra. 2018. International Symposium on Animal Production and Conservation for Sustainable Development

3. Probiotic Research in Several Products of Virgin Coconut Oil from Padang, Indonesia. 2018. Journal of Physic

Biography :

Yolani Syaputri is pursuing her PhD at Gifu University. She has worked in the area of especially food fermentation, lactic acid bacteria, and bacteriocins. Currently, she is developing fermented tea from Gifu, Japan. She is interested to find a potential lactic acid bacterium which is isolated from many samples. She focused on identification bacteriocin gene or structural gene, immunity protein and secretion gene to get the genetic information and develop them to make a good product.