Journal of Infectious Diseases and Treatment Open Access

Intestinal microbiota composition before and after probiotic treatment in dogs with diarrhoea

Joint Event on 6^th World Congress and Expo on Applied Microbiology & 8^th Edition of International Conference on Antibiotics, Antimicrobials & Resistance & 12^th International Conference on Allergy & Immunology

October 21-22, 2019 Rome, Italy

Ivana Kubasova, Lenka Micenkova, Jana Farbakova, Aladar Madar, Andrea Laukova and Viola Strompfova

Centre of Biosciences, Slovak Academy of Sciences, Slovakia Masaryk University, Czech Republic University of Veterinary Medicine and Pharmacy, Slovakia

Posters & Accepted Abstracts: J Infec Dis Treat

Abstract:

Gastrointestinal problems belong to top medical conditions diagnosed in dogs for several last years (5th place - diarrhoea/intestinal upset, 6th place vomiting/upset stomach in 2017; VPI, 2018). However antimicrobial drugs are still the most frequently used treatment option, probiotic bacteria together with electrolyte replacement are sufficient in many cases of diarrhoea without long-lasting disturbance of microbiota balance observed after antibiotic administration. Our intention was to test efficacy of probiotic supplementation (Enterococcus faecium DSM 32820 for 7 days, the dose 109 CFU/1 ml/dog/day) in dogs (n=6) with acute diarrhoea. The faecal microbiota was detected using 16S rRNA sequencing after DNA extraction using Qiagen DNeasy PowerLyzer PowerSoil Kit. Significant improvement of faecal consistency (faecal score) was observed after 7 days of probiotic supplementation while faecal pH values were not changed. The most abundant phylum Firmicutes was stable over time (80.2 % at day 0 and 77.1 % at day 7 in average). Less abundant phylum Proteobacteria was decreased (8.6 vs 3.5 %) whereas Bacteriodetes were increased (0.4 vs 6.1 %) after probiotic application. The most common family sequenced was the Lachnospiraceae representing 25.7 % of the total taxa identified. Among other detected families, Enterobacteriaceae (8.6 vs 3.4 %), Peptostreptococcaceae (19.6 vs 12.3 %) and Coriobacteriaceae (6.8 vs 0.3 %) were decreased. In contrast, Bifidobacteriaceae (0.3 vs 2.1 %), Prevotellaceae (0.3 vs 3.6 %) and Lachnospiraceae (24.4 vs 31.0 %) were more abundant at day 7 compared to day 0. The families Clostridiaceae and Lactobacillacea were not changed. At the genus level, predominant Lactobacillus genus was stable while Escherichia-Shigella was lower after probiotic treatment (8.4 vs 3.3 %).