Sachse C
EMBL Heidelberg, Germany
Posters & Accepted Abstracts: Biochem Mol biol J
Recently, we showed that autophagy receptor p62/SQSTM-1 assembles into flexible helical filaments. In the current talk, we provide further detailed insights into the molecular basis of polymer formation. Using EM based structure elucidation in vitro and in situ reveals large oligomeric and polymeric cargo receptor complexes giving rise to higher-order structures that constitute the scaffold for autophagosome formation. The organization of small receptor proteins into helical assemblies provides a cellular mechanism for high selectivity in cargo recognition and a fundamental architecture that enables cargo encapsulation of various sizes from molecular to cellular scale. The presented example illustrates the versatility and synergy of structural and cellular electron microscopy approaches. carsten.sachse@embl.de
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