Journal of Infectious Diseases and Treatment Open Access

A molecular characterization of Listeria monocytogenes from beef samples and cattle slaughterhouses located in federal district

Joint Event on 6^th World Congress and Expo on Applied Microbiology & 8^th Edition of International Conference on Antibiotics, Antimicrobials & Resistance & 12^th International Conference on Allergy & Immunology

October 21-22, 2019 Rome, Italy

Joana Marchesini Palma

University of Brasilia, Brazil

Posters & Accepted Abstracts: J Infec Dis Treat

Abstract:

The aim of the present work was the analysis of Listeria monocytogenes strains in beef samples as well as slaughterhouse environment, located in the federal district, promote serotyping by polymerase chain reaction (PCR), perform antibiotic susceptibility and submit the strains to Pulsed-field gel electrophoresis (PFGE). A total of 125 beef samples were analyzed, 45 samples of carcasses swabs and 43 swab samples. It detected 13 strains of Listeria monocytogenes, 11 in beef samples and 2 in slaughterhouse environmental. No carcass swabs strains were isolated. Among the 13 strains of Listeria monocytogenes, was found six strains of serotype 4b, five serotype 1/2c and two strains of serotype 1/2a. Among the 11 strains of Listeria monocytogenes found in beef, one (9.1%) strain showed resistance to erythromycin, one (9.1%) strain to gentamicin, one to ciprofloxacin (9.1%) and all strains (100%) were resistant to nalidixic acid. The two (2) strains coming from the slaughterhouse drains, all (100%) were resistant to nalidixic acid and Sulfonamides. The analysis by pulsed field gel electrophoresis (PFGE) showed 8 different pulsotypes, they were grouped into three different clonal groups, which coincidentally correlated with the three different serotypes found, what suggests a widespread dissemination of these profiles in the federal district.