Dr. Manish Mishra is pursuing his research at Kresge Eye Institute, Wayne State University, Michigan. His research focus is to understand the mechanism underlying the development of diabetic retinopathy and the complications associated with it. Diabetic retinopathy is a progressive disease, which affects blood vessels in the light-sensitive tissue and is the leading cause of vision impairment and blindness among working-age adults. Diabetic retinopathy is associated with increased free radical generation and mitochondrial damage coupled with cell apoptosis. Although several metabolic abnormalities have been associated with its development, due to complex nature of this multi-factorial disease, a link between any specific abnormality and diabetic retinopathy remains largely speculative. Mitochondria has its own genetic material that encodes 13 essential proteins required for proper functioning of Electron Transport Chain; dysfunctional mitochondria is a key source of cellular ROS and initiates cell death. In the development of diabetic retinopathy, his research group identified that reduced expression of mitochondrial DNA gene is one of the causal factors of mitochondrial dysfunction, reduced biogenesis and capillary cell death, one of target cells in the histopathology of retinopathy.
His current research objective is to investigate the mechanism/s behind increased oxidative stress and mitochondrial dysfunction and subsequent cell loss in the development of diabetic retinopathy