Department of Dentistry, Nihon University School of Dentistry, 1-8-13 Kanda Surugadai, Chiyoda-ku, Tokyo 1018310, Japan
Research Article
Stimulation of Transforming Growth Factor-?3 Signaling by Compressive Force in MC3T3-E1 Cells
Author(s): Moeko Togawa, Akira Nakajima*, Nichika Fukumashi, Hitoshi Kariya, Kotoe Mayahara, Takayuki Kawato and Mitsuru Motoyoshi
Objective: Expression of transforming growth factor (TGF)-β is strongly associated with osteoblast differentiation. However, the role of TGF-β3 in mediating the effects of compressive force as a mechanical stress on osteoblasts remains unclear. Here, we examined the effects of compressive force on the expression of TGF-β3, and downstream signaling pathways comprising inflammatory cytokines in osteoblasts. Effects of siRNA-mediated knockdown of TGF-β3 on Smad-dependent and Smadindependent signaling pathways, and on inflammatory cytokines were also examined.
Design: Cultured MC3T3-E1 osteoblast-like cells were subjected to a continuous compressive force (0.5, 1.0, or 2.0 g/cm2) for 30 min, 1 h, and 3 h. TGF-β3 expression was examined using real-time polymerase chain reaction and western blot analysis. Total/.. View More»
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