Department of Biological Sciences, Yarmouk University, Jordan
Review Article
Cell Delivery Strategies: Review of the Recent Developments, Challenges and Opportunities
Author(s): Ahmad M. Khalil*
Background: Integrating transgene into the host genome for stable maintenance in dividing cells can have unpredictable effects on gene expression and unintended effects on neighboring genes. Ethical concerns are particularly serious in the case of clinical applications using germ cells of heritable genome editing in humans, i.e., modifications introduced in sperm, eggs, or embryos to “create” genetically engineered children. One major challenge that faces in vivo gene editing (GE) technology is the selection of a suitable delivery route to transfer engineered DNA, RNA, or proteins into target cells. This problem originates from the large size of the key components and the low capacity of the delivery vector. In general, cell delivery methods available today can be broadly classified into three categories: chemical, biological and physical.
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