Pediatrics & Health Research Open Access

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Abstract

Serological Proteome Analysis for Identification of Potential Antigen in Atopic Dermatitis

Blendi Ura, Luigina De Leo, Giorgio Arrigoni, Federica Scrimin, Irene Berti and Tarcisio Not

Objective: Identify human skin derived auto antigens that could be recognized by IgE serum of patients with both severe atopic dermatitis and high serum IgE immunoglobulins.

Design: The study was performed retrospectively on sera collected from children with severe atopic dermatitis with total IgE serum concentration (IgE>3000 U.I./ml), as a serum sample controls we used the children’s sera suffering from severe food allergy and with high IgE concentrations against food proteins (IgE>90 kU/l). All these serum samples were used to identify skin-derived antigen by using immunoproteome technique.

Setting: Institute for Maternal and Child Health.

Patients: Six children suffering from severe atopic dermatitis and six suffering from severe food allergy without skin involvement.

Interventions: Serological proteome analysis (SERPA) for antigen identification.

Main outcome measures: First immunoproteomic study for identification of potential antigen in severe atopic dermatitis. Identification of two immunoreactive proteins IGKC (Ig kappa chain C region) and IGLC1 (Ig lambda-1 chain C regions).

Results: After membranes analysis we identified two immunoreactive proteins IGKC and IGLC1 present only in membrane treated with severe atopic dermatitis. Reaction of these proteins were considered non-specific event and didn’t further investigate.

Conclusions: Using SERPA analysis we identified two immuno-reactive proteins. Even these proteins have proved to be non-specific event; this showed the great potential of SERPA for antigen identification.