Department of General Surgery, University of Cape Town, South Africa
Original Research Article
Reaching the Remote: Dried Blood Spot Analysis for Disease Diagnosis on a Protein Microarray Platform
Author(s): Metoboroghene O. Mowoe*, Tristan Rensburg, Hisham Ali, Joshua Gqada, Urda Kotze, Marc Bernon, Bradley Africa, Eduard Jonas, Jonathan M. Blackburn
Context: Cancer remains one of the leading causes of death globally with an estimated 19.3 million cases and 10
million mortalities in 2020. In Africa and Asia, where remoteness is prevalent, access to healthcare facilities is limited,
providing a significant barrier to effective screening and early detection of cancers in at risk groups and thus,
incomplete registries.
Objectives: Here, we utilised low resource, low cost dried blood spots (DBS) based sample collection coupled with
robust, protein microarray technology to enable quantitative, multiplexed measurements of diagnostic auto antibody
biomarkers of disease, in minimal sample volumes. I
Methods: Specifically, we describe the development of a DBS extraction and elution method from low cost, homemade
blood cards. We then show that DBS stored at.. View More»
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