- (2012) Volume 4, Issue 4
Objective: To characterize the phytochemical constituents of Eupatorium triplinerve using GC – MS. Methods: Ten grams of the powdered sample was subjected to column chromatography over silica gel (100- 200 mesh) and eluted with n-hexane, chloroform, ethanol and methanol respectively. n-hexane and chloroform did not elute much of the compounds. The methanol fraction of the Eupharbatum triplinerve was taken for GC-MS analysis. The analysis was carried out on a GC Clarus 500 GC system with a column packed with Elite – 1 (10% dimethyl poly siloxane, 30 x 0.25 mm ID x 1 EM df), the compounds are separated using with Helium as carrier gas at a constant flow 1ml/min. sample extract (2 μL) injected into the instrument was detected by Turbo gold mass detector (Perkin Elmer) with the aid of the Turbo mass 5.1 software. Results: The GC MS analysis provided peaks of eleven different phytochemical compounds namely hexadecanoic acid (14.65%), 2,6,10-trimethyl,14-ethylene-14-pentadecne (9.84%), Bicyclo[4.1.0]heptane, 7-butyl- (2.38%), Decanoic acid, 8-methyl-, methyl ester (3.86%), 1-undecanol (7.82%), 1-hexyl-1-nitrocyclohexane (2.09%), 1,14-tetradecanediol (6.78%), Octadecanoic acid, 2-hydroxy-1,3-propanediyl ester (19.18%) and 2-hydroxy-3-[(9E) -9-octadecenoyloxy] propyl(9E)-9-octadecenoate (8.79%). Conclusion: The bioactive compounds in the methanolic extract of Eupatorium triplinerve have been screened using this analysis. Isolation of individual components would however, help to find new drugs.
Copyright: This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
