Journal of HIV & Retro Virus Open Access

Keywords

Cervical; Cancer; HIV; Cameroon

Introduction

The burden of disease in developing countries is being overwhelmed by known and emerging infections. It has also been shown by various studies that non-communicable diseases are becoming a public health problem in these communities [1]. Numerous viral infections have for long been associated with various types of cancers. Prominent amongst these is the relationship between the Human Papillomavirus (HPV) and cervical [1-3]. The history of HIV (Human Immuno-deficiency Virus) and its link with cancer started with the first descriptions of AIDS (Acquired Immuno-deficiency Syndrome) [4]. People infected with HIV have a higher risk of some types of cancer than uninfected people. Almost 4 decades after the apparition of the HIV/AIDS pandemic, new challenges to this disease are being faced by patients and the global health systems. Patients are living longer with HIV infection due to increase availability of HAART (Highly Active Anti-retroviral Therapy) with increased chances of developing a cancer [5].

Globally there are about 270,000 deaths from cervical cancer per annum with over 85% of these occurring in low resource countries, where it is the leading cause of cancer deaths in women [6,7]. Cameroon is rated as having and an age-standardized incidence rate of 87.3 cervical cancer cases per 100.000 persons [8].

There is still much controversy concerning the relationship between HIV and invasive cervical cancer. Globally, an increased risk of HPV infection and cervical pre-cancerous squamous intraepithelial lesions has been found in HIV-positive women [9,10]. In some European populations, like HPV, a positive association of HIV infection and invasive cancer of the cervix has also been documented [11,12]. However most studies in Africa on this association have been conflicting and inconclusive [13- 16]. While some of the studies infer that HIV infection could act as a cofactor for this disease in HPV infected women [17], others found no association between the two [18].

The interaction between HIV and host antigens and eventual occurrence of related cancers has not been fully understood. Due to immune compromise, cervical cancer in AIDS patients has peculiar characteristics different from that in immune competent patients [12]. If HIV infection increases both the likelihood of cervical cancer developing from dysplasia and the likelihood of a worsening of the stage of cervical cancer, a correlation could be expected between the HIV prevalence in Cameroon and the incidence of cervical cancer.

The national HIV infection rate in Cameroon has decreased from 12% in 1995 to 4.5% in 2014, yet there has been an upsurge of both AIDS-defining and other cancers in patients with the infection [19]. This is due to longer survival of HIV-infected patients which increases the risk of cancer amongst them. This occurs even though preventive actions against cervical cancer began two decades ago and culminated in 2014 with the launching of the National Programme of Vaccination against Human Papilloma Virus in Cameroon [20]. The advent of HIV/AIDS has caused a significant change in the epidemiology of cancer in Cameroon, reducing the average incidence age for the latter in the population by about 8 years [21].

In resource-stressed communities like ours, numerous difficulties are faced in the management of HIV/AIDS and cancer patients respectively, and the situation is worse in patients who have both diseases. The role of underlying genetics, co-infections, and lifestyle exposure to known/unknown antigens can never be underestimated, and therefore scientists should continue to investigate the interaction of HIV and its proteins with host machinery in the aetiology of oncogenesis from a molecular to epidemiologic level. This study of the profile of patients with co morbidity of HIV and cervical cancer in Cameroon is aimed at documenting basic epidemiologic and other findings that characterise patients with both diseases and serve as platform for further studies.

Materials and Methods

For this prospective study, data on patients with both HIV infection and cancer from 5 randomly selected pilot centres of an NCI (USA)-sponsored HIV malignancies project were assembled. The data collection began from January 1 to December 31, 2015. The patients were recruited at services in concerned with the management of cancer or HIV respectively. A patient who tested positive for the condition in one service was sent to the other service for the other specialized test. Patients who were found to be positive for both conditions were documented. It is from this cohort that we recruited patients who were positive for both HIV and a cervical cancer. Data on these patients was assembled and analyzed. Specifically we documented vital data on the patients with both co morbidities, data on HIV infection including mode of transmission, serotype, duration of infection and CD4 count. Concerning cancer we found out the morphology and extent of the disease. We then proceeded to cross-match these to find out if there was any association between them. Dates for incidence of the respective morbidities was inferred from the date of their respective diagnosis and HIV to cancer conversion time was calculated from date of diagnosis of HIV infection to date of diagnosis of associated cancer. It is from this cohort that we extracted data on patients who tested HIV positive and had a cervical cancer. The vital statistics of the patient, data on HIV on and data on the cancer were all assembled and analysed. The duration of both cancer occurrence and HIV infection were inferred from the respective dates of diagnoses of the diseases.

Results

Cervical cancer in HIV+ patients predominates at 2 peak age groups (30-39 years-27.3%) and (50-59 years-33.3%). A high proportion of cases are below 40 years of age (36.4%).

Majority (56%) of participants had a secondary education and beyond and 44% had not gone beyond primary school.

3 out of 10 regions of the country (30%) were covered by the study. Majority of patients (77.3%) were from the Center region. An annual average of 66 cases of HIV+/cervical Cancer cases was obtained.

Discussion

In Cameroon, breast and cervical cancers are the leading causes of cancer-related morbidity and mortality [20]. Cervical cancer is one of the AIDS-defining illnesses [22]. This is based on the observation that women infected with HIV had a significantly higher risk of developing Squamous Intraepithelial Lesions (SIL), the pre-malignant stage of cervical cancer, and a faster progression to more advanced stages of the diseases [23].

Other studies have also suggested [24], while others confirmed [25], yet some authors found an inconclusive correlation between malignant cervical lesions and HIV infection [26]. Nawal reported that Immune depression especially by HIV is known to be a risk factor for cervical cancer [7]. Innocent et al. hypothesized that HIV facilitates infection with specific types of HPV, some of which may promote (HPV-p types) or inhibit (HPV-i types) progression to cervical carcinogenesis in any single individual [22], while others [27] went as far as qualifying that HIV positive patients had 8.5 times risk to develop cervical cancer than their HIV negative counterparts.

We found 66 patients with co-morbid conditions of cervical cancer and infection with HIV in a one year prospective, crosssectional, multi-centre, pilot study in Cameroon (Table 1). This was more than previous reports [4] which found 36 cases in a ten year study in one hospital in Kenya. This disparity in incidence could be explained by the multiplicity of our study sources.

Table 1 Cervical cancer morphology amongst HIV+ patients.

Our patients ranged in age between 24 and 71 years at an average of 45.4 years and majority (33.3%) were in the 50-59 years age range (Figure 1). This finding was lower than a previous report [21] where a peak age of 50-59 years and an average of 55 years was found amongst HIV-naïve cervical cancer patients in Cameroon. Our finding was however similar to some [4] who found a mean age at diagnosis of cervical cancer of 47 years in HIV naïve patients. Sando [20] and Rhamadani [28] reported in a ten years study on trends of breast and cervical cancer in Cameroon and the influence of HIV infection on cervical cancer respectively that cervical cancer occurred in patients above 30 years of age.

Similar to our study, earlier reports in Zimbabwe showed that HIV-seropositive women with invasive cervical cancer were 5 years younger than HIV-negative women with invasive cervical cancer [29]. In the same trend Lomalisa et al. [30] reported that HIV-seropositive women in South Africa presented with invasive cervical cancer 10 years earlier than HIV-negative women. Most of our patients (58%) were aged 20-49 years, like Myeni who concluded in a similar study that HIV-positive cervical cancer patients were predominantly less than 49 years of age compared to their HIV negative counterparts [31].

In this study we covered 3 out of 10 regions of the country (30%); a ratio likely to impact on the national incidence of this disease (Table 2). Up to 69.7% of our patients were married (Figure 2). In consideration of HIV versus marital status, this rate is higher than the report by Shisana in South Africa who found HIV prevalence among married people of 10.5% compared to 15.7% among unmarried people. They inferred in their work that the risk of HIV infection did not differ significantly between married and unmarried people; and concluded that the relationship between marital status and HIV is complex, depending on various demographic factors and sex behaviour practices [32]. In a different study however, the author reported that those who were married living with their spouse had significantly reduced odds of being HIV-positive compared to all other marital spouses groups [33] We did not study marital sub groups in this work.

Table 2 Data sources for study on HIV+/ cervical cancer patients.

Considering the relationship between cervical cancer and marital status, the relative rarity of the disease in the unmarried and its higher incidence in formerly married than in currently married women had been reported with inference to association of the disease with number of sexual partners [34]. The same trend was reported by Sanae who attributed the protective effect of marriage from cervical cancer to be mostly at the level of prevention of the disease [35]. Myeni had a contrary finding in his study when he concluded that cervical cancer patients that were never married were 3.7 times more likely to be HIV positive than married patients [31].

The HIV serotype was predominantly type 1 (66.7%). This is higher than previous reports where an HIV-1 prevalence of 21.0% in a comparative study of HIV positive and negative women and concluded that HIV-1 infection is associated with invasive cancer of the cervix [36]. In 18.2% of our cases, infection was both by HIV serotypes 1 and 2. This is similar to an earlier report of 19.2% concurrent HIV-1/ HIV-2 infection in a study in Cameroon [37].

Concerning the route of contamination, it was mainly sexual for the cases where data was provided (Figure 3). This finding had been reported in previous studies [38].

In our study, majority (56%) of participants had a secondary education and beyond, while 44% had not gone beyond primary school (Figure 4). This is in contradiction to the common notion that cervical cancer is a disease of the lower class [39], assuming that education infers a high socio-economic status. Our finding is similar to the trend though lower in value to Hailemariam [27] who reported that the likelihood of cervical cancer among literate women was 6.1 times higher as compared to illiterate women at a value of 78.9% compared to the later.

In all cases (100%), the HIV infection preceded the apparition of cervical cancer for periods ranging between 1-6 years. The average duration of the infection before diagnosis of cancer was 2.5 years (Figure 5). Like in our study, some reports indicated the time interval since HIV/AIDS diagnosis amongst cervical cancer patients ranged from 1 month to 10 years, with a mean of 2.5 ± 2.2 years [40]. At the time of diagnosis most patients had CD4 counts between 200 and 500 cells/mm3 (66%) amongst cases whose counts were known at a mean of 400 cells/mm3 (Table 3). This is similar to an earlier report by where majority of cases (73.9%) had a CD4+ cell count between 200 and 500 cells/mm3 [40].

Table 3 CD4 count in HIV+/cervical cancer patients at time of cancer diagnosis.

Infection with HIV has been shown to be a risk factor for cervical cancer [41] and was also reported that HIV-positive women develop more cervical precancerous lesions compared with HIV negative women. The main pathological diagnosis found in our study involved the epithelial squamous cell of the cervix (Table 1). In 16.7% of our cases, the cervical lesion was a dysplasia (I, II or III). Earlier comparative studies had indicated that the prevalence of cervical precancerous lesions was higher (71.8%) in HIV-positive women than in HIV-seronegative women [28]. In this same study the prevalence of invasive cervical cancer was 8% in HIV-seronegative women and 11% in HIV-positive women, lower than our rate of 68.18%. Most of our patients were diagnosed with cancer at stage ≤ II (66.7%) (Figure 6). This is contrary to previous reports on stage of diagnosis of cancer from HIV naive screening studies in this community [21]. It is also contrary to a study in Zambia among HIV infected women which stated that not only does cervical cancer occur more in the immune suppressed, but HIV positive women also have higher likelihood of having high grade cervical squamous intra epithelial lesions [42]. Probably HIV infection promotes early presentation of patients to a health facility for checkups and consequently enables early screening and diagnosis of cervical cancer in HIV+ patients with various cervical lesions. This view had been expressed in an earlier report [21]. Unlike our finding some reports [4] found a lower rate of 44.6% cases of HIV/cervical cancer cases at stage ≤ II and concluded that there was no significant change in either age at presentation or severity of cervical cancer in HIV+ cervical cancer patients compared to HIV- counterparts. Though some reports have shown that the role of immunosuppression in the progression to invasive cancer may be marginal [43], we found a squamous cell lesion was the commonest histologic finding (84.84%) and a squamous cell carcinoma was most predominant, while in few cases the lesion was a dysplasia. 12.12% of these HIV-immune depressed patients had an adenocarcinoma of the cervix. This is lower than the finding that reported adenocarcinoma of the cervix to make up more than 20% of all cervical cancer cases diagnosed each year in the United States [44] and similar to others who found the rate to be above 12.5% [45].

Limitations of this Study

The present work has some limitations that should be considered in interpreting the results. First, the study participants were recruited from hospitals; thus, the findings might not be truly representative of HIV-infected women with cervical cancer who do not have access to hospitals. Second, this study was completed at 5 pilot sites and results cannot be generalized to all HIVpositive cervical cancer patients all over Cameroon. Third, the small sample size limited further analysis of factors that influence co morbidity of the 2 diseases. Fourth, the short duration could impact on our findings. Lastly, the information provided by the participants could be affected by social acceptability bias and/ or recall bias, despite our attempts to minimise this by ensuring anonymity.

Acknowledgements

The authors are grateful for the sponsorship by the New York University School of Medicine (NYUSoM) Daugherty Centre and the National Institute of Health (NIH) in the USA with Grant number D43CA153726.

Ethical clearance for the study was obtained from the Ministry of Public Health Ethical Review Board and the local Ethical Review Boards of the various institutions involved in the study.

Conflict of Interest

The authors declared no conflict of interest.

Contribution of Authors

All authors contributed in the conception, collection of data, write up, analysis, and proof reading of this article.

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