European Journal of Experimental Biology Open Access

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The study of effects common paraoxonase polymorphism (L55M) on atherosclerosis risk in diabetic patients by PCR-RFLP

Bayatmakoo Roshanak, Mobaiyen Haedeh, Monfaredan Amir, Katayoun Bahman Sofiani andRonak Benissy

Human serum paraoxonase1 (PON1) are proteins by molecular mass of 43 KDa(355 amino acids) that synthesis in liver and bind to high density lipoproteins (HDL). One of two polymorphism occurred in coding region of PON1 gene is substitution an amino acid of leucine (L) with methionine (M) at codon 55 which effects PON1 activities. On the other hand, most of diabetic patients' death is about atherosclerosis and its side effects. So, this study is conducted to study L55M polymorphism in type II diabetic patients referred to Shahid Madani Hospital, Tabriz, Iran. In this study, venous blood sampling was done on 95 diabetic (case group) and 105 non- diabetic (control group) with atherosclerosis patients. After DNA extraction and identification of quality of DNA, L55M mutation was assayed by using specific primers in PCR and byproduct of PCR was digested by Hinf1. From 200 samples that were taken from Iranian who lived in north-west of Iran, the 55LL, 55LM and 55MM genotypes in case group were 33.8%, 42.6% and 23.6% and in control group 29.9%, 48.3% and 21.8%, respectively. In several studies, relationship between presence of polymorphism and level of enzymatic variation were shown. These variations are introduced as a risk factor for heart and arterial disease and have an important role in incidence of atherosclerosis but in this study, there wasn't significant deference in frequency of polymorphism alleles by p value in 95% confidence index. These results showed that further studies must be done by more cases.