European Journal of Experimental Biology Open Access

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Study on prevalence of blood parasites of sheep and detection of their vectors using methyl green pyronin in Varamin, Iran

Mohammad Habibpour Tahamtan, Sedigheh Nabian, Mahdi Khodaveisi, Homan Ronaghi and Abbas Gerami Sadeghian

Theileria and Babesia are some major pathogens in animals which can transmit by ticks. They cause significant economic losses in tropical and sub-tropical regions of the world. Climate variability and its changes in different regions can change the parasites fauna and their vectors. So the regular study on their prevalence can be very useful for control and prevention strategies. For this purpose a total of 300 sheep with clinical sign (Fever, jaundice and anemia) in Varamin were tested for presence of Theileria and Babesia using Giemsa staining. Also the present ticks on these animals were collected and identified using the identification keys, and then salivary glands from some ticks collected from positive animals for presence of pathogens in blood were dissected and stained using Methyl Green Pyronin. Six tick species were identified as: Hyalomma anatolicum, H.asiaticum, H.marginayum, Rhipicephalus turanicus, Rh. bursa and Rh. sanguineus. Rhipicephalus female was the most frequent tick (51.87%) and Rh. sanguineus was the minor species (0.58%). In blood smears examination, Theileria was the only parasite which was seen with 2% frequency. None of the stained salivary glands had positive reaction to Methyl Green Pyronin that showed, positive animals for presence of Theileria, infected by another ticks previously. It seems that the life cycle of Theileria has not completed in collected ticks because of shortage of feeding period