European Journal of Experimental Biology Open Access

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Production of invertase enzymes from Saccharomyces cerevisiae strain isolated from sugarcane and grape juices

G. Arumugam, A. Mohamed Sadiq, M. Nagalingam and A. Panneerselvam

Aim of the present studySaccharomyces cerevisiaewas isolated from sugarcane and grape samples by using the standardize immobilization and non-immobilized technique. To test the sucrose hydrolysis activity ofimmobilized and non-immobilized form of Saccharomyces cerevisiae in sucrose broth, and quantitative and qualitative analysis of glucose by using Benedict’s method, Folin Wu method and Thin layer Chromatography. The isolated strain is better than the MTCC strain or similar then it could be possible to get a fresh stain for the industrial application at least after following strain improvement techniques. In conclusion the grape and sugarcane juice can be more potent used as invertase for the production of enzymes by using standard technique viz,immobilized and nonimmobilized.