European Journal of Experimental Biology Open Access

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Periodate oxidation studies of seeds polysaccharide from Wrightia tinctoria R.Br. (Roxb.) plant

R. B. Singh

Periodate oxidation is one of the most important reactions which is used in the structural study of non-ionic seeds polysaccharides in carbohydrate chemistry. Periodate oxidation was done using sodium metaperiodate as oxidant and it was proposed by Fluery & Lange’s method. The mole of periodate consumed during periodate oxidation reaction of seeds polysaccharide of Wrightia tinctoria R.Br. (Roxb.) was determined volumetrically. The composition and probable polysaccharide structure have also been elucidated with the information obtained from the periodate oxidation on purified fraction of seeds polysaccharide. It consumed 1.848 moles of oxidation and liberated 0.1452 moles of formic acid per mole of anhydrohexose sugar unit after 75 hrs.