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Isolation and characterization of sulphated polysaccharides from Codium tomentosum (J. Stackhouse, 1797) collected from southeast coast of India

Seedevi P., S. Sudharsan, S. Vasanth Kumar, A. Srinivasan, S. Vairamani and A. Shanmugam

A sulphated polysaccharide was isolated from Codium tomentosum by hot-water extraction, deproteinized, precipitation with ethanol, and obtained a crude polysaccharides yield from 24.2% based on dry weight source. The crude polysaccharides further fractionated by anion-exchange column chromatography using Q-Sepharose Fast Flow column, fractionation yielded was 17.31%, and purified by gel-permeation chromatography using Sephadex G-50 Fast Flow column they were yielded from 10.83% respectively. The biochemical composition of the sulphated polysaccharides contains total carbohydrate 88.7%, sulfate 14.7%, protein 0%, ash 15.9% and moisture 2.3% respectively. The FT-IR spectral analysis of the sulphated polysaccharides consist carboxyls and sulfate groups. The elemental analysis of sulphated polysaccharides possess higher amount of carbon at 25.86%, hydrogen 5.80%, nitrogen 6.50% and very less in sulfur at 0.16% respectively. The present study was concluded the sulphated polysaccharides would be in useful product development and bioprospecting approaches

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