European Journal of Experimental Biology Open Access

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Isolation and characterization of high salt tolerant bacteria from agricultural soil

Jaymin Mendpara, Vivek Parekh, Sudhir Vaghela, Atul Makasana, Prashant D. Kunjadia, Gaurav Sanghvi, Devendra Vaishnav and Gaurav S. Dave

Adaptation characteristic of microorganisms in different extreme conditions seeks discoveries in field of pharma, food, & bioenergy sectors. In the present study, new experimental approach was proposed to prove adaptation ability in extreme conditions with growth and development of agricultural soil bacterial species in stepwise adaptation in high salt (NaCl) conditions. Six different bacterial species were isolated from agricultural soil surrounding the Rajkot region. Out of six isolates, two showed salt tolerance up to 10% NaCl concentration. Biochemical and molecular (16S rDNA sequencing) characterization revealed the strains to be Exiguobacterium sp. and Serratia sp. designated as GSD1 and GSD 2 strains. Species were able to adapt upto 10% of NaCl in nutrient broth growth medium. Primary screening for extracellular enzymes (protease, amylase, lipase) secretion unveiled activity of enzymes in nutrient agar plates containing skim milk or starch or mineral oil for protease, amylase and lipase production respectively. This experiment provides the base to link the adaptation capabilities of agricultural soil microorganisms in high salty environment and vice a versa.