European Journal of Experimental Biology Open Access

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Identification of Candida Species Screened from Catheter Using Patients with PCR-RFLP Method

Alireza Farasat, Mohammad Ghahri, Hossein Mirhendi and Sedigheh Beiraghi

Candiduria or presence of infecting Candida species in urinary tract is one of the usual hospital infections. Factors increasing susceptibility of patients to such infections are including high utilization of antibiotics, long hospitalization periods, higher ages, diabetes mellitus, sexuality, female sex and use of immunosuppressive therapy. This study focuses not only on diagnosis of candiduria and its causes using phenotype and genotype analyzing methods, but also discusses on frequencies of Candiduria in catheter using patients. 250 samples of catheter using patients were collected and cultured in biological media. Identifying the yeasts, initially some phenotypic experiments were carried out such as germ tube, the production of hyphae or pseudohyphae and clamydospores in CMA+TW80 medium, and CHROMagar Candida. Genomic DNA of all species were extracted and analyzed with PCR and subsequent Polymerase Chain Reaction - Restriction Fragments Length Polymorphism (PCR-RFLP) methods. Analyzing urination of 95 female and 155 male, 40 samples were recognized as infected that demonstrates 55% and 45% Candiduria infection in females and males. Frequency of C. albicans, C. glabrata, C. tropicalis, C. parapsilosis, and C. krusei are 45%, 32.5%, 15%, 5%, and 2.5%, respectively. Candiduria frequency is measured to be about 16%.