European Journal of Experimental Biology Open Access

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Evaluation of clinical signs, gross lesions and antibody response in experimental of indvidual and co-infection of H9N2 avian influenza and Ornithobacterium rhinotracheale in SPF chickens

Aidin Azizpour, Hossein Goudarzi, MansourBanani, Abbas Nouri, RezaMomayez, Mohammad Hassan Hablolvarid, Mohammad Abdoshah and Peyman Bijanzad

In this study, clinical signs, gross lesions and antibody titer of (A/Chicken/Iran/ m.1/2010) H9N2 virus and (ORT - R87-7/1387) Ornithobacterium rhinotracheale bacteria alone and a co-infected group in SPF broiler chickens were investigated. Eighty 1-day-old specific pathogen-free White Leghorn chickens were randomly divided into four equal groups. At the age of three weeks, the chicks in the three experimental groups were inoculated by virus and bacteria, individually or concurrent and in control group allantoic fluid was inoculated. We used PCR for detection of virus in various organs of experimentally infected broiler.The results showed that experimental co-infection of AIV and ORT increased the severity of clinical signs, mortality rate and gross lesions. The HI titer against AIV infection in the co-infected group was higher than the HI titer of other groups, which may indicate that ORT could promote the propagation of H9N2 AIV or stimulate the immune response.