Adugnaw Admas*, Melaku Admassie, Tesaka Misgie, Berhane Kidane, Yigardu Mulatu
Oxytenanthera abyssinica A. Rich. Munro has various economic and ecological importances in Ethiopia. Nowadays, the need for planting the species across the wider landscape, as plantation forest, is increasing. However, conventional propagation methods of O. abyssinica are found generally to be inefficient because they have low multiplication rate, are time consuming, labor intensive and too costly. Accordingly, tissue culture protocol developed at Holeta biotechnology lab by the collaboration of the Forest Directorate of EIAR and Holeta biotechnology laboratory in 2017 for the species was later optimized at Bahir Dar-ORDA tissue culture laboratory in 2020 and 2021 so as to produce sufficient number of lowland bamboo seedling through seed culture. Murashige and Skoog (MS) medium augmented with 6-Benzylaminopurine (BAP) was used for shoot initiation and multiplication. MS medium supplemented with 3-Indole-Butric Acid (IBA) was used for in vitro rooting. In shoot initiation experiment all viable seeds were proliferated in 5-7 days of culturing. Shoot was successfully multiplied at 0.004 g/L BAP and best rooting response was found at 0.005 g/l IBA. The present optimized protocol enables actors who need large numbers of low land bamboo seedling for a forestation/reforestation programs.
Published Date: 2025-01-24; Received Date: 2023-08-07
