Saqib Ishaq*, Jamshaid Khan, Raheel Tahir, Abdul Aziz, Obaid Habib, Muhammad Inam Ul Haq, Karim Gul, Yasir Ali, Rehan Naeem
Mentha piperita, commonly known as peppermint, is a well-known medicinal plant with a range of therapeutic properties. In this study, we conducted an in-silico analysis of microRNAs and ESTs in Mentha piperita to gain insight into its genetic makeup. We obtained 29 EST sequences and 36 microRNAs from the NCBI database and analyzed them using various bioinformatics tools. Functional annotation of the microRNAs revealed that only 29% of the miRNAs met the criteria for AU content between 30% and 70%. Out of these, target genes were identified for only 9 sequences, which were annotated with basic roles in biological processes and molecular functions. Phylogenetic analysis confirmed the conservation of Mentha piperita with Arabidopsis thaliana. Additionally, we calculated E-values and energies for the ESTs and microRNAs, respectively. Our findings provide valuable insights into the genetic makeup of Mentha piperita and may aid in further understanding its therapeutic properties. Future research could involve the experimental validation of the predicted targets of the identified microRNAs and the characterization of the function of the ESTs.
Published Date: 2025-01-14; Received Date: 2023-09-19
