European Journal of Experimental Biology Open Access

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Characterization of a thermo tolerant lipase from Pichia anamola

Prakash Tiwari and Mukesh K. Upadhyay

A yeast strain of Pichia anamola was characterized for optimal production of liapse.The organism shown maximum enzyme production on 5th day of incubation. The optimum temperature and pH for th e enzyme activity was 45°C and 7.0 respectively. The enzyme r e t a i n e d 85 % activity at 55°C. Few metal ions like Ca+2 and Mg+2enhanced the activity whereas Fe+3, Hg+2and Ag+ declined the enzyme action. The enzyme was characterized by some inhibitors and solvents; amongst inhibitors, β – mercapto ethanol, SDS and urea had shown maximum inhibition where as amongst the solvents phenol and chloroform strongly repressed the activity.