Kalyani Saha*, Uzma Khan, Priyanka Basak, Sushmita Bhattacharya
According to WHO gastric cancer is fifth most common malignancies worldwide. Chronic Helicobactor pylori (H.P.) infection is one of common cause for gastric cancer. H.P. colonized in the human stomach, induces proinflammatory cytokines production, such as IL-1β, IL-8, IL-6, and (TNF)- α, which are closely associated with gastric cancer. HMGB1, mediates various inflammatory functions, e.g. promotes the activation of NLRP3 inflammasomes via NF-κB pathway. The interaction between HMGB1 and RAGE triggers NF-κB expression, which stimulates the release of pro- inflammatory cytokines, e.g. TNF-α, IL-8, IL-6, IL-1β that enhances the inflammatory response. H. pylori activates NLRP3 inflammasome which induces the signalling pathways through NFðÂ�?Â�?�?Â�?�?B, IL-1ðÂ�?Â�?�?½ production. NLRP3 senses a general signal that induces the sequential events of inflammasome activation and NF-κB regulates pro-inflammatory gene induction. Inhibitors against infectious bacteria like H.P. is quite challenging. Aims of the study was to reveal the effect a small herbal compound Caps. on H.P. infected gastric and macrophage cell line in relation to inflammatory responses of gastric cancer. H.P. culture, was used to infect gastric and macrophage cell line. Protein expression analysis showed that expression of HMGB1, NLRP-3 and (NF)-κB were high in H.P. treated group when compared with Caps treated group. Levels of pro- inflammatory cytokines were significantly inhibited in Caps treated group when compared with H.P. infected group in related cell lines and anti-inflammatory cytokines IL-10 was induced in Caps treated group when compared with H.P. infected group in related cell lines. It can be concluded that the small herbal compound Caps significantly inhibits Helicobacter Pylori induced inflammatory response in gastric and macrophage cell line in aspect of gastric cancer.
