Hossein Mostafavi, Ramin Farsadbakhsh and Gholamreza Zarrini
A diversity of biological activities and pharmaceutical uses have been attributed to phenolic acid derivatives such as antibacterial, anticancer, antiinflammatory (1-3). A series of 3,4,5-trihydroxy benzoic acid derivatives were synthesized and their structure confirmed by FT-IR, 1HNMR, 13CNMR, elemental analysis
In vitro biological activity of compounds was determined against proteus vulgaris ATCC 7829, Escherichia coli ATCC 25922, as (Gram-negative) bacteria and bacillus cereus ATCC 11778, Staphylococus aureus ATCC 6538 as (Gram-positive) bacteria. Antibacterial susceptibility tests were done by use of the paper disc diffusion method on Mueller Hinton agar (Merck). Chloramiphenicol, Penicilline, Streptomycin and Tetracycline were standard reference antibiotics. The zone of inhibition against bacteria was measured after 24 hours at 37°C. Compounds 3, 4, 5 were the main antibacterial compounds against Gram-negative bacteria but not Gram-positive.
